Department: Molecular Biology
Alternate Names for Test: PROTHROMBIN G20210A
Constituent Tests: FVL and Prothrombin Gene Mutation are performed together as a multiplex assay
Specimen Collection Requirements:
Phlebotomist Instructions: A single tube is adequate for both Factor V Leiden and Prothrombin G20210A testing, 
but cannot be shared with other tests.

NOTE: if patient is <16 years of age, then the requesting clinician must discuss 
with a haematologist to obtain approval before phlebotomy. Where approved / 
recommended, please provide the clinician's name and the clinical indication for 
the test in the clinical details section.

If the patient is willing to pay for the test, the patient's GP should phone the 
laboratory to seek approval from the Haematologist prior to having a sample 
collected. If the patient already has approval, then proceed with phlebotomy.
Sample Type:

1x Mauve (EDTA) tube 4.0mL

Minimum Sample Volume: 3 mL Whole blood. No separation - whole blood
Additional Information: Factor V Leiden is part of Thrombophilia screen.

Thrombophilia testing will be performed for the following clinical indications:

Idiopathic venous thrombo-embolism in young patients (<45 years) Recurrent 
Recurrent VTE despite adequate therapeutic anticoagulation
VTE in context of family history of unprovoked VTE in a first degree             
Thrombosis in unusual sites (e.g. cerebral, mesenteric, portal)               VTE 
in association with a history of thrombophlebitis
Warfarin-induced skin necrosis
Pregnancy complications such as pre-eclampsia, IUGR, implantation failure,  
placental abruption or recurrent miscarriage.*  

Thrombophilia screen will not be performed in the following clinical settings:

Prior to prescription of COC in patients with a personal history of VTE† 
Prior to prescription of COC in patients with a family history of VTE†
Arterial thrombosis (Lupus testing is indicated in this setting)

*   There is no evidence currently to support anticoagulation in women with 
pregnancy complications and a positive hereditary thrombophilia.  Trials are 
ongoing in this area.  However, it is recognised that in routine practice 
management decisions are currently made in conjunction with thrombophilia testing.

†   Current British guidelines recommend avoidance of the COC in this setting 
regardless of the thrombophilia results.

PLEASE NOTE:  As of 09.01.2012 Activated Protein C Resistance is no longer 
performed as part of a thrombophilia screen.

Referred Specimens:
Minimum Aliquot: 3mL
Pre-Transport Requirements to Reference Lab: Room temperature
Transport Requirements to Reference Lab: Room temperature
In the Lab:
Delphic Code: FVPT
Ultra Code: TMG
Testing Schedule: Fortnightly
Turnaround Time: 14 days
Method: LightCycler - PCR
Stability Time Limit for Add-On Tests: No add on testing possible
Link to Application and Diagnostic Use Website: https://www.rcpa.edu.au/Library/Practising-Pathology/RCPA-Manual/Items/Pathology-Tests
Contact Details:
Lab Contact: Chor Ee Tan
Department Name: Molecular Biology
Phone: (04) 245 0239

Test Information last updated on 15/08/2017
Website last updated on 15/01/2018 07:29

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