|FACTOR V (5) LEIDEN|
|Alternate Names for Test:||
|Constituent Tests:||FVL and Prothrombin Gene Mutation are performed together as a multiplex assay|
|Specimen Collection Requirements:|
|Phlebotomist Instructions:||A single tube is adequate for both Factor V Leiden and Prothrombin G20210A testing,
but cannot be shared with other tests.
NOTE: if patient is <16 years of age, then the requesting clinician must discuss
with a haematologist to obtain approval before phlebotomy. Where approved /
recommended, please provide the clinician's name and the clinical indication for
the test in the clinical details section.
If the patient is willing to pay for the test, the patient's GP should phone the
laboratory to seek approval from the Haematologist prior to having a sample
collected. If the patient already has approval, then proceed with phlebotomy.
1x Mauve (EDTA) tube 4.0mL
|Minimum Sample Volume:||3 mL Whole blood. No separation - whole blood|
|Additional Information:||Factor V Leiden is part of Thrombophilia screen.
Thrombophilia testing will be performed for the following clinical indications:
Idiopathic venous thrombo-embolism in young patients (<45 years) Recurrent
Recurrent VTE despite adequate therapeutic anticoagulation
VTE in context of family history of unprovoked VTE in a first degree
Thrombosis in unusual sites (e.g. cerebral, mesenteric, portal) VTE
in association with a history of thrombophlebitis
Warfarin-induced skin necrosis
Pregnancy complications such as pre-eclampsia, IUGR, implantation failure,
placental abruption or recurrent miscarriage.*
Thrombophilia screen will not be performed in the following clinical settings:
Prior to prescription of COC in patients with a personal history of VTE†
Prior to prescription of COC in patients with a family history of VTE†
Arterial thrombosis (Lupus testing is indicated in this setting)
* There is no evidence currently to support anticoagulation in women with
pregnancy complications and a positive hereditary thrombophilia. Trials are
ongoing in this area. However, it is recognised that in routine practice
management decisions are currently made in conjunction with thrombophilia testing.
† Current British guidelines recommend avoidance of the COC in this setting
regardless of the thrombophilia results.
PLEASE NOTE: As of 09.01.2012 Activated Protein C Resistance is no longer
performed as part of a thrombophilia screen.
|Pre-Transport Requirements to Reference Lab:||Room temperature
|Transport Requirements to Reference Lab:||Room temperature
|In the Lab:|
|Turnaround Time:||14 days|
|Method:||LightCycler - PCR|
|Stability Time Limit for Add-On Tests:||No add on testing possible|
|Link to Application and Diagnostic Use Website:||https://www.rcpa.edu.au/Library/Practising-Pathology/RCPA-Manual/Items/Pathology-Tests|
|Lab Contact:||Chor Ee Tan|
|Department Name:||Molecular Biology|
|Phone:||(04) 245 0239|
Test Information last updated on 15/08/2017
Website last updated on 15/01/2018 07:29